The Forum Confocal Microscopy Core
Equipment: Andor BC43 CF Confocal Microscope
Applications
The system supports fixed cells and tissue mounted on standard microscope slides as well as imaging in plate formats.
Live-cell imaging is not supported.
Plate formats (6, 12, 24, and 96 wells) can be imaged using 2x, 10x, 20x, and 60x air objectives with automatic stage control on imaging-grade plates.
Fusion Software
System control and image acquisition via Fusion software.
Integrated deconvolution.
Laser Lines
Excitation lines: 405 nm, 488 nm, 561 nm and 638 nm.
Objectives
2x air objective 0.06NA 7.5mm WD.
10x Plan Apo air Objective 0.45NA 4mm WD.
20x Plan Apo air Objective 0.75NA 1mm WD.
60x Plan Apo L air Objective 0.95NA 0.21mmWD.
60x Plan Apo L oil Objective 1.4NA 0.13mm WD.
Camera Specs
4.1 MP (6.5μm pixel, 16-bit) monochrome sCMOS camera up to 82% QE. With a 18.4 mm field of view.
| Internal AU Health | Other Public Institiutions | Private Companies | |
|---|---|---|---|
| Training (including 1 hour) | 1.130 DKK | 1.690 DKK | 5.650 DKK |
| Hourly Price | 220 DKK | 290 DKK | 960 DKK |
| Staff Support per Hour | 400 DKK | 560 DKK | 1.690 DKK |
Pricing is subject to change
Full service available upon request
Workflow-Specific Requirements and Additional Services
Users who wish to work with plate formats must book a separate training session.
Histopathological evaluation of routine stains (e.g. H&E, PAS, Masson trichrome) may be available and is performed by a pathology-trained MD. Requests are considered on a case-by-case basis against a fee.
Location: Forum Aarhus University Hospital, Palle Juul-Jensen’s Blvd. 11, 8200 Aarhus N.
User Eligibility
PhD students, postdocs, staff and senior researchers are eligible.
Undergraduate students may be granted access upon special agreement.
Requests for academic collaborations are evaluated on a case-by-case basis.
If you have not taken a microscopy course or lack significant experience, you must complete lessons 2, 3, 5, and 6 from the Andor Microscopy Training Course.
If you plan to perform deconvolution, please also complete lesson 11.
To request access, please contact
Professor Lene Niemann Nejsum
nejsum@clin.au.dk